Using a narrow band, frequency-doubled output of a pulsed- dye-amplifier, simultaneous absorption and laser-induced fluorescence measurements of OH have been performed to study the rotational dependence of the collisional quenching of the OH fluorescence emission from 2(Sigma) + levels due to water molecules. In these studies, the OH was produced in a low pressure water vapor microwave discharge cell. The microwave power was varied in the range between 30 to 50 Watts in order to investigate variations in the quenching rate, translational, and rotational temperatures with microwave power. From the absorption lineshape data, it was determined that, for a given microwave setting, the OH molecules in the region of excitation are in both rotational and transnational thermal equilibrium. The quenching results indicate a slight variation with increasing microwave power. They also show a variation with the excited rotational level. For K > 4, the quenching rate decreases as the rotational level excitation increases. This is in agreement with previously observed trends. Finally, the quenching rate data obtained in this study are compared to those available in the literature and discrepancies are discussed.