29 December 1997 Alteration of mitochondrial membrane potential in photosensitized cardiocytes monitored by fast-response fluorescent probe
Author Affiliations +
Abstract
Mitochondria constitute well-established target for photodynamic action provided that duration of incubation time with hydrophobic photosensitizer is long enough (18 - 24 hrs) to enable incorporation of the dye into these organelles. It was found that 1 hour incubation of rat cardiocytes with hematoporphyrin dihydrochloride (Hp) followed by low-energy HeNe laser irradiation (ED: 0.3, 1.0, or 3.0 J/cm2), affected mitochondrial membrane potential. The latter was monitored by means of the fast-response fluorescent probe DASPMI and the dynamic video imaging system equipped with frame-grabbing and analyzing software. The time-course of mitochondrial membrane potential decrease exhibited dependence on both: Hp concentration and ED used. In cells incubated with 1 (mu) g/ml Hp a decrease of DASPMI fluorescence occurred during 8 - 15 mins and was preceded by oscillations of fluorescence intensity. For cells incubated with 100 (mu) g/ml Hp disappearance of discrete pattern of mitochondrial fluorescence was observed 1 - 2 mins after irradiation.
© (1997) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Mariusz Adamek, Asher Shainberg, Fidi Schwartz, Shimon Rochkind, "Alteration of mitochondrial membrane potential in photosensitized cardiocytes monitored by fast-response fluorescent probe", Proc. SPIE 3191, Photochemotherapy: Photodynamic Therapy and Other Modalities III, (29 December 1997); doi: 10.1117/12.297796; https://doi.org/10.1117/12.297796
PROCEEDINGS
9 PAGES


SHARE
Back to Top