Kinetics of porphyrin partitioning in cells and membranes: investigation of cholesterol effects

Vladimir P. Zorin; Iosif S. Mikhalovsky; Tatyana E. Zorina

doi:10.1117/12.297823

29 December 1997 Kinetics of porphyrin partitioning in cells and membranes: investigation of cholesterol effects

Vladimir P. Zorin, Iosif S. Mikhalovsky, Tatyana E. Zorina

Proceedings Volume 3191, Photochemotherapy: Photodynamic Therapy and Other Modalities III; (1997) https://doi.org/10.1117/12.297823
Event: BiOS Europe '97, 1997, San Remo, Italy

Abstract

Insertion of cholesterol into phosphatidylcholin vesicles modifies the kinetics of intermembrane porphyrin movement due to as its effects on the rate of transmembrane and intermembrane pigment transfer, as the change of pigment intramembrane distribution pattern. The cholesterol-induced alteration of porphyrin intra- and intermembrane redistributions depends strongly on pigment polarity. According to flow cytometric study results, the reduction of cellular cholesterol through the incubation of cells with phosphatidylcholin liposomes enhances the cells' affinity for porphyrins and at that time efficiently promoted pigments clearance from cells. The potential role for cholesterol and its disproportionation within the cellular membranes as determinants of porphyrin binding and retention by cells is suggested.

Citation Download Citation

Vladimir P. Zorin, Iosif S. Mikhalovsky, and Tatyana E. Zorina "Kinetics of porphyrin partitioning in cells and membranes: investigation of cholesterol effects", Proc. SPIE 3191, Photochemotherapy: Photodynamic Therapy and Other Modalities III, (29 December 1997); https://doi.org/10.1117/12.297823

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