Photodynamic nerve cell killing: dynamics of electrophysiological responses and comparison of photosensitizers

Anatoly B. Uzdensky

doi:10.1117/12.297794

29 December 1997 Photodynamic nerve cell killing: dynamics of electrophysiological responses and comparison of photosensitizers

Anatoly B. Uzdensky

Author Affiliations +

Proceedings Volume 3191, Photochemotherapy: Photodynamic Therapy and Other Modalities III; (1997) https://doi.org/10.1117/12.297794
Event: BiOS Europe '97, 1997, San Remo, Italy

Abstract

The study of single neuron response to photodynamic effect provides evaluation of the dynamics of cytotoxic events leading from initial threshold changes to the cell death and allows us to compare phototoxicity of different substances. The photosensitized isolated crayfish mechanoreceptor neurons were irradiated with the He-Ne laser (632.8 nm, 0.3 W/cm²), and their responses were recorded. The following photosensitizers were studied: methylene blue, janus green B, protoporphyrin IX, chlorins e6 and p6 and sulphonated aluminum phthalocyanine 'Photosens.' Crayfish neurons were insensitive to the He-Ne laser irradiation and photosensitization alone, but very sensitive to photodynamic effect: they changed firing rate and died at nanomolar concentrations of photosensitizers. The dynamics of neuron responses depended on the photosensitizer type and its concentration. Dependencies of firing acceleration rate and neuron lifetime on photosensitizer concentration allowed to compare efficiencies of different photosensitizers. Photosens and chlorin p6 were found to be the most potent photosensitizers.

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Anatoly B. Uzdensky "Photodynamic nerve cell killing: dynamics of electrophysiological responses and comparison of photosensitizers", Proc. SPIE 3191, Photochemotherapy: Photodynamic Therapy and Other Modalities III, (29 December 1997); https://doi.org/10.1117/12.297794

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