Photosensitization of tumor cells: are comparable conditions an impossible dream?

Joerg G. Moser; Tatyana A. Karmakova; Sonja Andrees; Birgit Wagner

doi:10.1117/12.297798

29 December 1997 Photosensitization of tumor cells: are comparable conditions an impossible dream?

Joerg G. Moser, Tatyana A. Karmakova, Sonja Andrees, Birgit Wagner

Proceedings Volume 3191, Photochemotherapy: Photodynamic Therapy and Other Modalities III; (1997) https://doi.org/10.1117/12.297798
Event: BiOS Europe '97, 1997, San Remo, Italy

Abstract

Photodynamic therapy (PDT)-induced kinetics of apoptosis were studied in V79 cells using several differently localized photosensitizing dyes, mostly tetraphenylporphine derivatives. Apoptotic fractions were quantified by flow cytometry after staining the samples by the terminal deoxynucleotidyl transferase (TdT)-assay. Methylene blue derivative (MBD), a new dye for PDT, and 5-aminolevulinic acid (ALA)-induced protoporphyrin IX that are both localized in mitochondria, induced apoptosis rapidly within hours after PDT. With MBD it was shown that rapid apoptosis was induced only with dye concentration above a certain threshold. With a lower dye concentration apoptosis was delayed more than one day and was induced due to inhibition of oxidative phosphorylation. After PDT with two membrane localized dyes, tetra(3- hydroxyphenyl)porphyrin (3THPP) and Photofrin, maximal induction of apoptosis took about 12 h. With two lysosomal localized sulfonated meso-tetraphenylporphines (TPPS_2a and TPPS₄) no apoptosis was induced until more than 12 h after PDT. The results are discussed in relation to evidence in the literature on the nature of possible pathways involved.

Citation Download Citation

Joerg G. Moser, Tatyana A. Karmakova, Sonja Andrees, and Birgit Wagner "Photosensitization of tumor cells: are comparable conditions an impossible dream?", Proc. SPIE 3191, Photochemotherapy: Photodynamic Therapy and Other Modalities III, (29 December 1997); https://doi.org/10.1117/12.297798

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