Different PS application methods for laserlight-induced fluorescence diagnostics (LIFD) of dysplasia and early cancer of the colon

Johannes Gahlen; Jochen Pressmar; Josef Stern; J. Boehm; Hanns-joerg Sinn; I. A. Born; R. Holle; Christian Herfarth

doi:10.1117/12.297849

22 December 1997 Different PS application methods for laserlight-induced fluorescence diagnostics (LIFD) of dysplasia and early cancer of the colon

Johannes Gahlen, Jochen Pressmar, Josef Stern, J. Boehm, Hanns-joerg Sinn, I. A. Born, R. Holle, Christian Herfarth

Author Affiliations +

Proceedings Volume 3192, Medical Applications of Lasers in Dermatology, Ophthalmology, Dentistry, and Endoscopy; (1997) https://doi.org/10.1117/12.297849
Event: BiOS Europe '97, 1997, San Remo, Italy

Abstract

Conventional diagnostics fail to trace dysplasias and early cancers of the colon. By means of Laserlight Induced Fluorescence Diagnostics (LIFD) macroscopically not visible malignant tumors and dysplasias of the colon can be detected. The main side effect of systemically applicated photosensitizers is a general photosensibilization of the organism which provokes an increased photosensitivity of the body. Local application of (delta) -aminolevulinic acid (ALA) reduces systemic uptake without impairment of the quality of investigation. Colorectal cancer in male wistar rats was induced by weekly subcutaneous (s.c.) injections of 1,2- dimethylhydrazine (DMH) for 17 weeks. After s.c. DMH injection the wistar rats (n equals 25) developed in average 5.6 macroscopically visible colon carcinomas. 80% of these tumor were detected by LIFD after photosensibilization with Photofrin II intravenously (i.v.), 29% after application of mTHPC (i.v.), 13% after ALA (i.v.), 20% after intraperitoneal application of ALA (i.p.) and 57% after local application of ALA in the colon. LIFD is a capable method for the detection of macroscopically not visible dysplasias and colon carcinomas of the rat induced by DMH. The applied PS and the method of application influences the detection of carcinomas and dysplasias. Systemic photosensibilization of the whole organism can be avoided by the local application of ALA without limiting the quality of investigation.

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Johannes Gahlen, Jochen Pressmar, Josef Stern, J. Boehm, Hanns-joerg Sinn, I. A. Born, R. Holle, and Christian Herfarth "Different PS application methods for laserlight-induced fluorescence diagnostics (LIFD) of dysplasia and early cancer of the colon", Proc. SPIE 3192, Medical Applications of Lasers in Dermatology, Ophthalmology, Dentistry, and Endoscopy, (22 December 1997); https://doi.org/10.1117/12.297849

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