Low-coherence in-depth microscopy for biological tissue imaging: design of a real-time control system

Loic Blanchot; Martial Lebec; Emmanuel Beaurepaire; Philippe Gleyzes; Albert Claude Boccara; Herve Saint-Jalmes

doi:10.1117/12.301057

1 January 1998 Low-coherence in-depth microscopy for biological tissue imaging: design of a real-time control system

Loic Blanchot, Martial Lebec, Emmanuel Beaurepaire, Philippe Gleyzes, Albert Claude Boccara, Herve Saint-Jalmes

Proceedings Volume 3194, Photon Propagation in Tissues III; (1998) https://doi.org/10.1117/12.301057
Event: BiOS Europe '97, 1997, San Remo, Italy

Abstract

We describe the design of a versatile electronic system performing a lock-in detection in parallel on every pixel of a 2D CCD camera. The system is based on a multiplexed lock- in detection method that requires accurate synchronization of the camera, the excitation signal and the processing computer. This device has been incorporated in an imaging setup based on the optical coherence tomography principle, enabling to acquire a full 2D head-on image without scanning. The imaging experiment is implemented on a modified commercial microscope. Lateral resolution is on the order of 2 micrometers , and the coherence length of the light source defines an axial resolution of approximately 8 micrometers . Images of onion cells a few hundred microns deep into the sample are obtained with 100 dB sensitivity.

Citation Download Citation

Loic Blanchot, Martial Lebec, Emmanuel Beaurepaire, Philippe Gleyzes, Albert Claude Boccara, and Herve Saint-Jalmes "Low-coherence in-depth microscopy for biological tissue imaging: design of a real-time control system", Proc. SPIE 3194, Photon Propagation in Tissues III, (1 January 1998); https://doi.org/10.1117/12.301057

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