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13 January 1998 Antigen recognition by T-lymphocyte studied with an optical trap
Xunbin Wei, Tatiana B. Krasieva, Paul A. Negulescu, Zhanxiang Zhang, Chung-Ho Sun, Michael W. Berns, Gregory J. Sonek, Michael D. Cahalan, Bruce J. Tromberg
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Proceedings Volume 3196, Optical and Imaging Techniques for Biomonitoring III; (1998) https://doi.org/10.1117/12.297930
Event: BiOS Europe '97, 1997, San Remo, Italy
Abstract
T-cell contact with antigen-presenting B cells initiates an activation cascade which includes an increase in T-cell intracellular calcium and leads to T-cell proliferation and differentiation. We studied cell-cell contact requirements for T-cell activation using an optical trap to control the orientation of T-cell/B-cell pairs and fluorescence microscopy to measure subsequent T-cell(Ca2+)i response. B cells or beads coated with antibodies to the T- cell receptor are trapped with a titanium-sapphire laser and placed at different locations along the T-cell, which has a polarized appearance defined by the shape and direction of crawling. T-cell (Ca2+)i is detected as an emission shift from the combination of fura-red and oregon- green, two cytoplasmic (Ca2+)i indicators. T- cells which are presented antigen at the leading edge have a higher probability of responding and a shorter latency of response than those contacting B-cells or beads with their trailing end.
© (1998) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
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Xunbin Wei, Tatiana B. Krasieva, Paul A. Negulescu, Zhanxiang Zhang, Chung-Ho Sun, Michael W. Berns, Gregory J. Sonek, Michael D. Cahalan, and Bruce J. Tromberg "Antigen recognition by T-lymphocyte studied with an optical trap", Proc. SPIE 3196, Optical and Imaging Techniques for Biomonitoring III, (13 January 1998); https://doi.org/10.1117/12.297930
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KEYWORDS
Optical tweezers
Calcium
Luminescence
Microscopy
Receptors
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