29 December 1997 Colon cancer diagnosis using fluorescence spectroscopy and fluorescence imaging technique
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It is well known that fluorescence spectroscopy can provide information about the differences in the concentration of chromophores in healthy and cancerous tissues. The tumor detection potential can be enhanced by using exogenous fluorescent agents with selective accumulation in cancerous tissue. In this study healthy and cancerous human colon tissue samples were obtained after colon surgery. Excitation-emission matrices were collected using a fluorescence spectrometer. The optimum excitation wavelength lied at 340 nm. After the acquisition of autofluorescence spectra, the samples were incubated in a solution of 4 (mu) g/ml of Rhodamine analogs. Rhodamine B, Rhodamine 6G and three recently synthesized analogs, were used. For the acquisition of fluorescence images, an endoscopic imaging system was developed. Fluorescence imaging with the concomitant use of Rhodamine analogs revealed a remarkable differentiation of cancerous from healthy colonic mucosa.
© (1997) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Dido M. Yova, Dido M. Yova, Vassilis Atlamazoglou, Vassilis Atlamazoglou, P. Davaris, P. Davaris, Nikolaos Kavantzas, Nikolaos Kavantzas, Spyros Loukas, Spyros Loukas, } "Colon cancer diagnosis using fluorescence spectroscopy and fluorescence imaging technique", Proc. SPIE 3197, Optical Biopsies and Microscopic Techniques II, (29 December 1997); doi: 10.1117/12.297946; https://doi.org/10.1117/12.297946

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