29 December 1997 Fluorescence microscopy image deconvolution: application to anthracycline distribution in breast cancer cells
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Abstract
Video epifluorescence microscopy and image analysis are used for studying anthracycline resistance in breast cancer cells. In order to perform a semi-quantitative image analysis, several deconvolution algorithms are tested and validated on model beads. The most performant algorithm is applied to fluorescent biological specimens. We show that deconvolution makes image segmentation easier. Semi-quantitative measurements on resulting images are correlated with results obtained by cytometry.
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Marie-Pierre Gramain, Corinne Bour, Alain Chomik, Alain Dieterlen, Olivier Haeberle, Jean Jacques Meyer, Sophie Marchal, Jean-Louis Merlin, Francois H. Guillemin, "Fluorescence microscopy image deconvolution: application to anthracycline distribution in breast cancer cells", Proc. SPIE 3197, Optical Biopsies and Microscopic Techniques II, (29 December 1997); doi: 10.1117/12.297960; https://doi.org/10.1117/12.297960
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