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29 December 1997 Near-field optical microscopy of bacteria thin sections
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Abstract
Whole bacteria as well as thin sections were investigated in our laboratory by means of near field scanning optical microscope (NSOM). The main problem in NSOM operation is a control of distance between microscopy tip and sample surface. The bacteria thin section is a more preferable sample for NSOM investigation because of its flat surface. For increasing of thin sections' image contrast were used different light microscopy stainers (Eosin, Hematoxylin, etc.). We obtained images of thin sections of plague (Y. Pestis EV) and cholera (V. Cholerae). Lateral resolution in the investigation is about 300 angstroms.
© (1997) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Nikolai P. Konnov, Vil B. Baiburin, Anatolyi A. Shcherbakov, Alina N. Malakhaeva, and Yuri P. Volkov "Near-field optical microscopy of bacteria thin sections", Proc. SPIE 3197, Optical Biopsies and Microscopic Techniques II, (29 December 1997); https://doi.org/10.1117/12.297978
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