Spectral luminescence analysis of amniotic fluid

Ekaterina I. Slobozhanina; Nataly M. Kozlova; Leonid P. Kasko; Marina V. Mamontova; Eugene A. Chernitsky

doi:10.1117/12.297958

Abstract

It is shown that the amniotic fluid has intensive ultra-violet luminescence caused by proteins. Along with it amniotic fluid radiated in the field of 380 - 650 nm with maxima at 430 - 450 nm and 520 - 560 nm. The first peak of luminescence ((lambda) _exc equals 350 nm; (lambda) _em equals 430 - 440 nm) is caused (most probably) by the presence in amniotic fluid of some hormones, NADH₂ and NADPH₂. A more long-wave component ((lambda) _exc equals 460 nm; (lambda) _em equals 520 - 560 nm) is most likely connected with the presence in amniotic fluid pigments (bilirubin connected with protein and other). It is shown that intensity and maximum of ultra-violet luminescence spectra of amniotic fluid in normality and at pathology are identical. However both emission spectra and excitation spectra of long-wave ((lambda) greater than 450 nm) luminescence of amniotic fluid from pregnant women with such prenatal abnormal developments of a fetus as anencephaly and spina bifida are too long-wave region in comparison with the norm. Results of research testify that spectral luminescent analysis of amniotic fluid can be used for screening of malformations of the neural tube. It is very difficult for a practical obstetrician to reveal pregnant women with a high risk of congenital malformations of the fetus. Apart from ultrasonic examination, cytogenetic examination of amniotic fluid and defumination of concentrations of alpha-fetoprotein and acetylcholin-esterases in the amniotic fluid and blood plasma are the most widely used diagnostic approaches. However, biochemical and cytogenetic diagnostic methods are time-consuming. In the present work spectral luminescence properties of the amniotic fluid are investigated to determine spectral parameters that can be used to reveal pregnant women with a high risk of congenital malformations of their offsprings.

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Ekaterina I. Slobozhanina, Ekaterina I. Slobozhanina, Nataly M. Kozlova, Nataly M. Kozlova, Leonid P. Kasko, Leonid P. Kasko, Marina V. Mamontova, Marina V. Mamontova, Eugene A. Chernitsky, Eugene A. Chernitsky, "Spectral luminescence analysis of amniotic fluid", Proc. SPIE 3197, Optical Biopsies and Microscopic Techniques II, (29 December 1997); doi: 10.1117/12.297958; https://doi.org/10.1117/12.297958

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