Time-resolved energy transfer spectroscopy for measuring mitochondrial metabolism in living cells

Herbert Schneckenburger; Michael H. Gschwend; Wolfgang S. L. Strauss; Reinhard Sailer; Manfred Bauer; Rudolf W. Steiner

doi:10.1117/12.297964

29 December 1997 Time-resolved energy transfer spectroscopy for measuring mitochondrial metabolism in living cells

Herbert Schneckenburger, Michael H. Gschwend, Wolfgang S. L. Strauss, Reinhard Sailer, Manfred Bauer, Rudolf W. Steiner

Proceedings Volume 3197, Optical Biopsies and Microscopic Techniques II; (1997) https://doi.org/10.1117/12.297964
Event: BiOS Europe '97, 1997, San Remo, Italy

Abstract

Energy transfer from NADH to the mitochondrial marker rhodamine 123 (R123) was used to probe mitochondrial malfunction of cultivated endothelial cells incubated with various inhibitors of specific enzyme complexes of the respiratory chain. Pronounced differences of 'energy transfer efficacy' of incubated cells as compared to controls were deduced from the ratio of fluorescence intensity and intracellular amount of the acceptor. A combination of cw and time-gated (nanosecond) fluorescence spectroscopy appeared to be an appropriate tool for probing mitochondrial malfunction in various kinds of diseases.

Citation Download Citation

Herbert Schneckenburger, Michael H. Gschwend, Wolfgang S. L. Strauss, Reinhard Sailer, Manfred Bauer, and Rudolf W. Steiner "Time-resolved energy transfer spectroscopy for measuring mitochondrial metabolism in living cells", Proc. SPIE 3197, Optical Biopsies and Microscopic Techniques II, (29 December 1997); https://doi.org/10.1117/12.297964

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