22 December 1997 Enhancing growth of cultured human skin cells using low-energy CO2 laser
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Abstract
In view of the versatility and usage of the CO2 laser as a too. in surgery and dermatology, we have studied its effect on enhancing proliferation of cultured skin cells using an attenuated CO2 laser. Exposure of cultured keratinocytes or fibroblasts to continuous wave or pulse mode irradiation enhanced thymidine incorporation by 1.4 to 1.7 folds, and cell number by 1.25 to 1.4 folds, measured 24 and 48 hours later, depending on the fluency applied. As expected, these effects were not suppressed by added antioxidants, indicating that the mechanism involved in this newly observed effect, differ from photosensitization by low energy visible and near IR lasers.
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Nili Grossman, Haim Reuveni, Sima Halevy, Rachel Lubart, "Enhancing growth of cultured human skin cells using low-energy CO2 laser", Proc. SPIE 3198, Effects of Low-Power Light on Biological Systems, (22 December 1997); doi: 10.1117/12.298002; https://doi.org/10.1117/12.298002
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