Laser tweezers as novel nonlinear tools in cell and biomolecular diagnostics

Karsten Koenig

doi:10.1117/12.301127

1 January 1998 Laser tweezers as novel nonlinear tools in cell and biomolecular diagnostics

Karsten Koenig

Proceedings Volume 3199, Biomedical Systems and Technologies II; (1998) https://doi.org/10.1117/12.301127
Event: BiOS Europe '97, 1997, San Remo, Italy

Abstract

In cell and molecular biology, single-beam gradient force optical traps (`laser tweezers') are mainly used as 3D micromanipulation tools, e.g. in controlled cell transport. Otherwise, near infrared (NIR) laser tweezers may act as useful diagnostic tools. They can be used (1) for sensitive measurements on highly-motile cells or organelles by sample confinement within the focal volume, (2) as force transducer, and (3) as linear and nonlinear excitation source. In the case of cells with one-photon NIR absorbers, such as erythrocytes, linear NIR excitation of hemoglobin can result in photothermal hemolysis. In addition, two- photon excitation of a variety of endogenous cellular absorbers, such as NAD(P)H, flavin and porphyrins, occurs de to the high photon flux densities in optical traps. Two- photon NIR excited visible fluorescence of endogenous as well as within a sub-femtoliter focal volume allows a novel nonlinear fluorescence diagnostics of cells and biomolecules.

Citation Download Citation

Karsten Koenig "Laser tweezers as novel nonlinear tools in cell and biomolecular diagnostics", Proc. SPIE 3199, Biomedical Systems and Technologies II, (1 January 1998); https://doi.org/10.1117/12.301127

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
5 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Near infrared

Optical tweezers

Luminescence

Diagnostics

Absorption

Continuous wave operation

Molecular biology

Show All Keywords

Keywords/Phrases

Search In:

Publication Years