Development of a model to evaluate laser penetration in the equine using the Nd:YAG laser as a standard

Lloyd P. Tate V.D.M.; Anthony T. Blikslager; Paul E. Mickel

doi:10.1117/12.312311

1 July 1998 Development of a model to evaluate laser penetration in the equine using the Nd:YAG laser as a standard

Lloyd P. Tate V.D.M., Anthony T. Blikslager, Paul E. Mickel

Proceedings Volume 3245, Lasers in Surgery: Advanced Characterization, Therapeutics, and Systems VIII; (1998) https://doi.org/10.1117/12.312311
Event: BiOS '98 International Biomedical Optics Symposium, 1998, San Jose, CA, United States

Abstract

The Nd:YAG laser is a frequently used laser in correcting equine upper respiratory disorders. Evaluation of this laser and several other lasers ability to penetrate tissue has been based on in vitro studies using portions of the arytenoid cartilage. The parameters measured have routinely been depth and width of crater created from irradiation of tissue. This investigation was performed on cadaver acquired tissue with anticipation of developing a model to conduct future in vivo studies of a similar nature to evaluate and compare different lasers. To perform this power setting, tissue selection and means of acquiring measurements needed to be standardized. Due to its accessibility and anatomic similarity to the arytenoid used in previous studies the rostral nasal septum was chosen as the second tissue for comparison with the arytenoid. Evaluation of the selected energies delivered to the tissues by the Nd:YAG laser were evaluated to set a standard by determining depth and top and bottom diameters of the area ablated. Methods of measurement consisting of standard histologic preparation followed by microscopic evaluation was compared to computer tomography acquired determinations. Statistical analysis supported the hypothesis that the nasal septum is a viable substitute for the arytenoid cartilage for future in vivo studies. It is also easily accessible compared to the arytenoid and evaluation of laser induced lesion parameters either in situ or separate from the animal should not be detrimental to the animal.

Citation Download Citation

Lloyd P. Tate V.D.M., Anthony T. Blikslager, and Paul E. Mickel "Development of a model to evaluate laser penetration in the equine using the Nd:YAG laser as a standard", Proc. SPIE 3245, Lasers in Surgery: Advanced Characterization, Therapeutics, and Systems VIII, (1 July 1998); https://doi.org/10.1117/12.312311

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