Spectroscopic study of the effect of laser heating on collagen stability: implications for tissue welding

Ward Small IV; Luiz Barroca Da Silva; Dennis L. Matthews

doi:10.1117/12.312298

1 July 1998 Spectroscopic study of the effect of laser heating on collagen stability: implications for tissue welding

Ward Small IV, Luiz Barroca Da Silva, Dennis L. Matthews

Proceedings Volume 3245, Lasers in Surgery: Advanced Characterization, Therapeutics, and Systems VIII; (1998) https://doi.org/10.1117/12.312298
Event: BiOS '98 International Biomedical Optics Symposium, 1998, San Jose, CA, United States

Abstract

Collagen is believed to play a major role in laser tissue welding. Furthermore, the fundamental fusion mechanism(s) may include thermal denaturation of the collagen fibers. An in vitro investigation of the effect of laser heating (1.9 micrometer diode laser) on collagen conformation was performed. Fourier transform infrared (FTIR) spectra of native and heated porcine cornea were obtained. A low-frequency shift in the amide A band of collagen around 3320 cm^-1 indicated that conformational changes associated with denaturation occurred. Samples heated using a temperature feedback controlled 1.9 micrometer diode laser showed a gradual decrease in the amide A peak frequency with increasing temperature, as did samples heated in a water bath. Complete denaturation was achieved at temperatures above 85 degrees Celsius for both heating protocols. Water loss induced by laser heating may have reduced the mobility of the collagen polypeptide chains, and contributed to the elevated denaturation temperature.

Citation Download Citation

Ward Small IV, Luiz Barroca Da Silva, and Dennis L. Matthews "Spectroscopic study of the effect of laser heating on collagen stability: implications for tissue welding", Proc. SPIE 3245, Lasers in Surgery: Advanced Characterization, Therapeutics, and Systems VIII, (1 July 1998); https://doi.org/10.1117/12.312298

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