Characterization of atherosclerotic lesions with laser-induced time-resolved fluorescence spectroscopy

Jean-Michel I. Maarek; Laura Marcu; Warren S. Grundfest M.D.

doi:10.1117/12.305377

16 April 1998 Characterization of atherosclerotic lesions with laser-induced time-resolved fluorescence spectroscopy

Jean-Michel I. Maarek, Laura Marcu, Warren S. Grundfest M.D.

Proceedings Volume 3250, Optical Biopsy II; (1998) https://doi.org/10.1117/12.305377
Event: BiOS '98 International Biomedical Optics Symposium, 1998, San Jose, CA, United States

Abstract

The time-dependent decay of arterial wall fluorescence remains relatively unexplored. We measured the timeresolved fluorescence emission (337 urn excitation) of 37 excised samples of human aorta classified histologically as normal or Type I- Type Va atherosclerotic lesion (Stary's classification). Time-resolved spectra (370-510 nm, 0.5 ns temporal resolution) were constructed by deconvoluting the emission waveform using the discrete-time Laguerre basis for expansion of the decay. The spectra changed markedly with tissue type, being spectrally broad for normal samples and peaked for atherosclerotic samples. In the time dimension, the emission lasted longer for the atherosclerotic samples. Biexponential approximation of the fluorescence time-dependent decay (390 nm) showed that the long decay constant for Type II lesions (5.95±0.16 ns) was larger than the corresponding parameter for normal arterial wall (5.42±0.17 ns) and smaller than the decay constant for intermediate (Type III) and advanced (Types P1-Va) lesions (6.88±0.32 ns, 7.38±0.51 ns). Discriminant analysis using the time constants at 390 nm and the intensity ratio I 39011 470 as predictor variables classified near-normal (normal-Type I) and diseased samples (Types ffi-V) with <90% sensitivity. 75% of Type II lesions were correctly classified. These results suggest that atherosclerotic lesions can be accurately classified based on their time-resolved fluorescence spectra. Keywords: time-resolved fluorescence, atherosclerosis, nitrogen laser, arterial wall, classification of lesions

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Jean-Michel I. Maarek, Laura Marcu, and Warren S. Grundfest M.D. "Characterization of atherosclerotic lesions with laser-induced time-resolved fluorescence spectroscopy", Proc. SPIE 3250, Optical Biopsy II, (16 April 1998); https://doi.org/10.1117/12.305377

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