Time-resolved laser-induced fluorescence of lipids involved in development of atherosclerotic lesion lipid-rich core

Laura Marcu; Jean-Michel I. Maarek; Warren S. Grundfest M.D.

doi:10.1117/12.305375

16 April 1998 Time-resolved laser-induced fluorescence of lipids involved in development of atherosclerotic lesion lipid-rich core

Laura Marcu, Jean-Michel I. Maarek, Warren S. Grundfest M.D.

Author Affiliations +

Proceedings Volume 3250, Optical Biopsy II; (1998) https://doi.org/10.1117/12.305375
Event: BiOS '98 International Biomedical Optics Symposium, 1998, San Jose, CA, United States

Abstract

The goal of this study is to define the spectro-temporal fluorescence emission of the main types of lipoid that accumulate in atherosclerotic lesions cholesterol esters: cholesteryl linoleate (CL) and cholesteryl oleate (CO). CO is commonly found in fatty-streaks whereas CL and FC predominate in advanced lesions and their necrotic core. Fluorescence emission of the purified components was induced with Nitrogen laser pulses and detected with a MCP-PMT connected to a digital oscilloscope. Using a Laguerre expansion of kernels algorithm the intrinsic fluorescence pulses were deconvolved from the measured transient pulses. A double exponential function depicted the fluorescence decay characteristics.

Citation Download Citation

Laura Marcu, Jean-Michel I. Maarek, and Warren S. Grundfest M.D. "Time-resolved laser-induced fluorescence of lipids involved in development of atherosclerotic lesion lipid-rich core", Proc. SPIE 3250, Optical Biopsy II, (16 April 1998); https://doi.org/10.1117/12.305375

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