Translator Disclaimer
18 May 1998 Effect of laser irradiation on the functional activity of enzymes with different structural complexity
Author Affiliations +
Proceedings Volume 3255, Applications of Ultrashort-Pulse Lasers in Medicine and Biology; (1998) https://doi.org/10.1117/12.308213
Event: BiOS '98 International Biomedical Optics Symposium, 1998, San Jose, CA, United States
Abstract
Three enzymes differing in their structural composition were irradiated by UV lasers to study the effect of temperature, protein concentration and addition of small molecules on their sensitivity to radiation exposure. The laser-induced effects were due to the structural complexity of the protein molecules and depended on the dose applied, the wavelength and the density of irradiation. The multi-enzyme 2- oxoglutarate dehydrogenase complex was subjected to pronounced irradiation-induced changes whereas the response of the two other enzymes was less significant. Reduction of the protein levels in irradiated samples was important under the XeCl laser coercion and the effects depended on the doses applied. The laser irradiation effects are suggested to be realized by means of conformational changes in the protein molecules and intermolecular association- dissociation processes.
© (1998) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Svetlana A. Ostrovtsova, Alexander P. Volodenkov, Alexander A. Maskevich, Irina M. Artsukevich, Slavomir S. Anufrik, Alexander F. Makarchikov, Ivan P. Chernikevich, and Vitali I. Stepuro "Effect of laser irradiation on the functional activity of enzymes with different structural complexity", Proc. SPIE 3255, Applications of Ultrashort-Pulse Lasers in Medicine and Biology, (18 May 1998); https://doi.org/10.1117/12.308213
PROCEEDINGS
7 PAGES


SHARE
Advertisement
Advertisement
Back to Top