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1 May 1998 Differential two-color x-ray radiobiology of membrane/cytoplasm in yeast cells and lymphocytes
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Proceedings Volume 3256, Advances in Optical Biophysics; (1998)
Event: BiOS '98 International Biomedical Optics Symposium, 1998, San Jose, CA, United States
An overall approach into the differential investigation of membrane/cytoplasm related metabolism and of cell-cycle of yeast cells after two color soft X-ray irradiation is presented; the soft X-rays being generated in trains of picosecond pulses by laser-plasma interaction. The two color X-ray differential technique is based on the generation of approximately 0.6 KeV X-rays which are deposited only in the membrane-wall complex switching off the anaerobic (fermentative) activity of yeast cells and on the generation of approximately 1.2 KeV X-rays which are mainly deposited in the cytoplasm, mitochondria and nucleus of yeast cells, mainly affecting the aerobic metabolism. A synergetic analysis of the metabolism is discussed, mainly founded on the recording of different correlated metabolic parameters, both on-line and delayed. Among the relevant access, pressure monitoring in batch samples acquire a dominant role allowing the identification of metabolic oscillation, that represent a marker of physical and chemical actions performed on the samples at different times. The experience acquired on yeast cells metabolism is being used to investigate lymphocytes metabolism and the related oscillatory properties of relevant enzymatic complexes. Actually even if it is not exactly the same as the mammalian situation, it should really propel the whole field forward.
© (1998) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Marziale Milani, Monica Ballerini, Giuliana Baroni, Dimitri Batani, Simona Cozzi, Lorenzo Ferraro, Michele Costato, Francesca Salsi, Achille Pozzi, Franco Squadrini, I. C. Edmond Turcu, Ric M. Allott, Nicola Lisi, Waseem Shaikh, and Shaun Hughes "Differential two-color x-ray radiobiology of membrane/cytoplasm in yeast cells and lymphocytes", Proc. SPIE 3256, Advances in Optical Biophysics, (1 May 1998);

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