Fluorescence anisotropy controlled by light quenching

Ignacy Gryczynski; Zygmunt Gryczynski; Joseph R. Lakowicz

doi:10.1117/12.307076

1 May 1998 Fluorescence anisotropy controlled by light quenching

Ignacy Gryczynski, Zygmunt Gryczynski, Joseph R. Lakowicz

Proceedings Volume 3256, Advances in Optical Biophysics; (1998) https://doi.org/10.1117/12.307076
Event: BiOS '98 International Biomedical Optics Symposium, 1998, San Jose, CA, United States

Abstract

We demonstrated that fluorescence anisotropy can be effectively decreased or increased in the presence of light quenching, depending on relative polarizations of excitation and quenching pulses. For parallel light quenching anisotropy decreases to 0.103 and z-axis symmetry is being preserved. In the presence of perpendicular light quenching, the steady- state anisotropy of pyridine 2 glycerol solution increases from 0.368 for unquenched sample to 0.484, for quenched one. We show that angular distribution of transition moments loses the z-axis symmetry in the presence of perpendicular light quenching. In these cases we used more general definitions of anisotropy. Induced by light quenching anisotropy can be applied in both, steady-state and time-resolved measurements. In particular, the systems with low or none anisotropy can be investigated with proposed technique.

Citation Download Citation

Ignacy Gryczynski, Zygmunt Gryczynski, and Joseph R. Lakowicz "Fluorescence anisotropy controlled by light quenching", Proc. SPIE 3256, Advances in Optical Biophysics, (1 May 1998); https://doi.org/10.1117/12.307076

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