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1 May 1998 Multiphoton excitation of fluorescence
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Proceedings Volume 3256, Advances in Optical Biophysics; (1998) https://doi.org/10.1117/12.307075
Event: BiOS '98 International Biomedical Optics Symposium, 1998, San Jose, CA, United States
Abstract
The increasing availability of fs lasers has resulted in expanded interest in multi-photon excitation of fluorescence. In this overview paper, we describe recent developments in multi-photon induced fluorescence spectroscopy and anisotropy. Experimental results are presented for three-photon excitation of tyrosine and tryptophan, and for the single tryptophan protein, troponin C Mutant F22. Three-photon excitation has been also applied to study DPH-labeled membranes and DNA stained with DAPI. The enhancement in spatial resolution resulting from three-photon excitation in spectroscopic and microscopic conditions is described for BBO scintillator in solution. We also describe two-photon excitation with using two-photon at different wavelengths.
© (1998) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Joseph R. Lakowicz and Ignacy Gryczynski "Multiphoton excitation of fluorescence", Proc. SPIE 3256, Advances in Optical Biophysics, (1 May 1998); https://doi.org/10.1117/12.307075
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