Probing the structure of macromolecules using microsecond time-resolved fluorescence of europium chelates

Ewa Heyduk; Tomasz Heyduk

doi:10.1117/12.307063

1 May 1998 Probing the structure of macromolecules using microsecond time-resolved fluorescence of europium chelates

Ewa Heyduk, Tomasz Heyduk

Proceedings Volume 3256, Advances in Optical Biophysics; (1998) https://doi.org/10.1117/12.307063
Event: BiOS '98 International Biomedical Optics Symposium, 1998, San Jose, CA, United States

Abstract

Lanthanide chelates, due to their unique luminescence properties, offer a potential for improvement and simplification of fluorescence resonance energy transfer distance measurements. In this report we present a procedure for incorporation of highly-luminescent europium chelate into internal sites of ds DNA. Using this labeling strategy donor- acceptor labeled 20 bp DNA fragments were prepared containing a stretch of six A residues (A-tract) in the middle of the fragment. Distance measurement between the donor (europium chelate) and acceptor (Cy5) were performed and compared to measurements with a DNA fragment lacking the A-tract. Only small changes in measured distances were observed between these two DNA molecules. The implications of these results for existing models of A-tract induced DNA bending are discussed.

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Ewa Heyduk and Tomasz Heyduk "Probing the structure of macromolecules using microsecond time-resolved fluorescence of europium chelates", Proc. SPIE 3256, Advances in Optical Biophysics, (1 May 1998); https://doi.org/10.1117/12.307063

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