24 April 1998 Evaluation of fiber optic probes for in-vivo Raman spectroscopy
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Proceedings Volume 3257, Infrared Spectroscopy: New Tool in Medicine; (1998); doi: 10.1117/12.306104
Event: BiOS '98 International Biomedical Optics Symposium, 1998, San Jose, CA, United States
Abstract
Raman spectroscopy has been sued for the analysis of biological tissue. Preliminary studies, which have been performed ex vivo, indicate that potentially useful diagnostic information may be obtained from the spectra. A new fiber optic-based in vivo Raman system has been constructed which can obtain spectra in vivo from tissue in less than 30 s. Unfortunately, tissue spectroscopy is hindered by the fluorescence and Raman signal generated in the silica fiber optic cables that are used for delivery of the excitation light and collection of the scattered light from the sample. Various fiber optic probes have been evaluated to quantify the extent to which each is able to suppress the fiber optic fluorescence and silica Raman during in vivo measurements. These included (1) standard 'cut-end' bifurcated silica probes, (2) large probe head attachments that utilized filters to eliminate the fiber signal and (3) fiber optic probes which have optimized collection efficiencies and use 'in-the-tip' (ITT) filters to eliminate the spectral contamination. The collection efficiency and the fiber optic suppression capabilities of these probes were compared. Signal response functions were measured and tissue spectra were collected to evaluate the performance of each probe.s
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Martin G. Shim, Brian C. Wilson, Eric Marple, Michael L. Wach, "Evaluation of fiber optic probes for in-vivo Raman spectroscopy", Proc. SPIE 3257, Infrared Spectroscopy: New Tool in Medicine, (24 April 1998); doi: 10.1117/12.306104; https://doi.org/10.1117/12.306104
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KEYWORDS
Raman spectroscopy

Fiber optics

Luminescence

Tissue optics

Tissues

Optical filters

Silica

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