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24 April 1998 Interpretation and processing of NIR spectra of turbid biological tissue
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Proceedings Volume 3257, Infrared Spectroscopy: New Tool in Medicine; (1998)
Event: BiOS '98 International Biomedical Optics Symposium, 1998, San Jose, CA, United States
NIR spectra of biological tissue consist of a number of broad, overlapping absorbance bands on a sloping vaseline. The interpretation and processing of such spectra are complicated by multiple-scattering interactions that distort the shapes of the absorbance bands and introduce wavelength- dependent scattering losses. In this paper we explain the dependence of the shape of the diffuse-reflection log(1/R) spectrum of a turbid medium on the scattering coefficient and probe geometry. From measurements on tissue phantoms and biological tissue, we observe that the separation distance between source and detector probes affects the sensitivity of the reflectance to changes in the density of scattering centers and alters the wavelength dependence of the baseline slope of the log(1/R) spectra. A new method, called fractional derivative processing (FDP), is introduced for extracting information from broad absorption bands corrupted by residual baseline variations and high-frequency noise. FDP was evaluated on spectra obtained from living tissue and tissue phantoms. Possible applications include NIR spectroscopy of hemoglobin, water, and other absorbers in human skin.
© (1998) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Joseph M. Schmitt, Hua Yang, and Jianan Y. Qu "Interpretation and processing of NIR spectra of turbid biological tissue", Proc. SPIE 3257, Infrared Spectroscopy: New Tool in Medicine, (24 April 1998);

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