As biosensors become more sophisticated and commercially available, the general appreciation for their capabilities also increases. We now focus on multi-analyte sensors and address the problems inherent in discriminating multiple simultaneous signals without loss in assay speed, specificity or sensitivity. Furthermore, the goals of portability, simplicity and low cost have not diminished in importance. NRL is developing a multi-analyte sensor designed to be portable, inexpensive, and easy to use. To achieve these goals, we use a room temperature CCD, a diode laser, and a disposable waveguide. While our goals of using automated fluidics and automated image processing are not yet completely realized, we have fabricated a prototype biosensor which fits into a tackle box with a associated portable computer. Simple microscope slides are used as waveguides and precoated with arrays of immobilized antibodies. Fluorescence immunoassays are performed on these waveguides using as many as 6 samples at a time and assaying for up to 5 different analytes in each samples Sensitivities in the 1-10 ng/ml range have been achieved in 10-minute assays. Initial studies in clinical fluids indicate that assays can be run on samples such as whole blood, plasma, urine, saliva and nasal secretions.