High-performance microfabricated capillary array electrophoresis systems for DNA analysis

Peter C. Simpson; Richard A. Mathies

doi:10.1117/12.304377

26 March 1998 High-performance microfabricated capillary array electrophoresis systems for DNA analysis

Peter C. Simpson, Richard A. Mathies

Proceedings Volume 3258, Micro- and Nanofabricated Structures and Devices for Biomedical Environmental Applications; (1998) https://doi.org/10.1117/12.304377
Event: BiOS '98 International Biomedical Optics Symposium, 1998, San Jose, CA, United States

Abstract

The microfabrication of DNA sample preparation, electrophoretic separation and detection devises is making possible a new generation of high-speed, high-throughput DNA analysis systems. HIgh quality DNA fragment sizing and short tandem repeat analyses have been preformed on microfabricated capillary electrophoresis devices that are 10-100 times faster than conventional electrophoretic separations. Microfabricated capillary array electrophoreses (CAE) plates increase the throughput of these devices by permitting the analysis of multiple samples in parallel. The practical utility of these devices has been improved by developing elastomer sample well and electrode arrays with standard microtiter dish spacings. We initially developed a CAE microplate that can analyze 48 samples using an array of 12 capillaries. More recently, we have developed an improved microplate with an array of 48 capillaries capable of analyzing up to 96 DNA samples in < 8 minutes. These highly parallel, miniaturized DNA analysis systems are a significant step toward fast and inexpensive genetic analysis.

Citation Download Citation

Peter C. Simpson and Richard A. Mathies "High-performance microfabricated capillary array electrophoresis systems for DNA analysis", Proc. SPIE 3258, Micro- and Nanofabricated Structures and Devices for Biomedical Environmental Applications, (26 March 1998); https://doi.org/10.1117/12.304377

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available