Microfabricated devices for sizing DNA and sorting cells

Hou-Pu Chou; Charles Spence; Axel Scherer; Stephen R. Quake

doi:10.1117/12.304378

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26 March 1998 Microfabricated devices for sizing DNA and sorting cells

Hou-Pu Chou, Charles Spence, Axel Scherer, Stephen R. Quake

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Proceedings Volume 3258, Micro- and Nanofabricated Structures and Devices for Biomedical Environmental Applications; (1998) https://doi.org/10.1117/12.304378
Event: BiOS '98 International Biomedical Optics Symposium, 1998, San Jose, CA, United States

Abstract

We have microfabricated devices to size and sort microscopic biological objects, ranging from cells to single molecules of DNA. Sizing is accomplished by fluorescent excitation and detection. The devices are fabricated in a silicone elastomer using a replica method. Single molecules of DNA have been sized to 10 percent accuracy, and manipulation of E. Coli cells has been demonstrated.

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Hou-Pu Chou, Charles Spence, Axel Scherer, and Stephen R. Quake "Microfabricated devices for sizing DNA and sorting cells", Proc. SPIE 3258, Micro- and Nanofabricated Structures and Devices for Biomedical Environmental Applications, (26 March 1998); https://doi.org/10.1117/12.304378

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