Miniaturization of multifunctional biosensors with enzyme-immobilized beads

Yuji Murakami; Kenji Yokoyama; Eiichi Tamiya

doi:10.1117/12.304381

26 March 1998 Miniaturization of multifunctional biosensors with enzyme-immobilized beads

Yuji Murakami, Kenji Yokoyama, Eiichi Tamiya

Proceedings Volume 3258, Micro- and Nanofabricated Structures and Devices for Biomedical Environmental Applications; (1998) https://doi.org/10.1117/12.304381
Event: BiOS '98 International Biomedical Optics Symposium, 1998, San Jose, CA, United States

Abstract

We propose a novel method for individual immobilization. Biomaterials were first immobilized enlarges the size of biomaterials to the size of sensor element. Then the support is arranged on the sensor element by self-assembling. The element was microfabricated to have a microstructure for self-assembling. When both size of biomaterial and the element are the same, self-assemble is expected to give one- to-one correspondence, and then individual response. Mixed glass beads immobilized each of various enzyme were put near the pits fabricated one chip and the chip was slanted to roll the beads into the pits. When the beads immobilized only with peroxidase were arranged, the addition of luminol and hydrogen peroxide gave chemiluminescence at almost every site. Next, the beads immobilized only with glucose oxidase as dummy enzyme were mixed with HRP-beads and arranged to the sites. Addition of substrate gave limited number of luminescence-giving sites, though every site had an enzyme- immobilized bead. These results show that two kinds of enzymes were separately arranged in the site in one-to-one correspondence.

Citation Download Citation

Yuji Murakami, Kenji Yokoyama, and Eiichi Tamiya "Miniaturization of multifunctional biosensors with enzyme-immobilized beads", Proc. SPIE 3258, Micro- and Nanofabricated Structures and Devices for Biomedical Environmental Applications, (26 March 1998); https://doi.org/10.1117/12.304381

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