Surface-plasmon-resonance-based array biosensors for multianalyte detection

Michael J. O'Brien II; Victor H. Perez-Luna; Leonard M. Tender; Mark Edmunds; Ben Lascelles; Steven R. J. Brueck; Gabriel P. Lopez

doi:10.1117/12.304386

26 March 1998 Surface-plasmon-resonance-based array biosensors for multianalyte detection

Michael J. O'Brien II, Victor H. Perez-Luna, Leonard M. Tender, Mark Edmunds, Ben Lascelles, Steven R. J. Brueck, Gabriel P. Lopez

Author Affiliations +

Proceedings Volume 3258, Micro- and Nanofabricated Structures and Devices for Biomedical Environmental Applications; (1998) https://doi.org/10.1117/12.304386
Event: BiOS '98 International Biomedical Optics Symposium, 1998, San Jose, CA, United States

Abstract

Surface plasmon resonance (SPR) is a phenomenon wherein the reflectance versus angle-of-incidence profile for a thin gold film illuminated with p-polarized light has a distinct minimum at a particular angle. This minimum of reflectance is due to an absorption of the light energy by the surface electron plasma of the metal occurring when the surface- parallel components of the light and plasmon propagation vectors match up. The value of this particular angle of incidence changes in proportion to the degree of adsorption of analytes to the metal film. This allows SPR to be used as a simple, noninvasive, optical tool for measuring the binding of chemical analytes. With a predetermined pattern of chemically specific receptors bound to the gold film, it is possible to detect a variety of species and concentrations of analytes, provided that one has a sensor platform capable of resolving the different reactions in each element of the receptor array. We have developed such a platform which is capable of optically monitoring an array of analyte receptors immobilized on gold coated microscope slides in real time. Moreover, the optical resolution of sensor platform allows the receptors to be micro-patterned.

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Michael J. O'Brien II, Victor H. Perez-Luna, Leonard M. Tender, Mark Edmunds, Ben Lascelles, Steven R. J. Brueck, and Gabriel P. Lopez "Surface-plasmon-resonance-based array biosensors for multianalyte detection", Proc. SPIE 3258, Micro- and Nanofabricated Structures and Devices for Biomedical Environmental Applications, (26 March 1998); https://doi.org/10.1117/12.304386

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