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29 April 1998 Cell permeabilization and molecular transport by laser microirradiation
Tatiana B. Krasieva, Curtis F. Chapman, Vickie J. LaMorte, Vasan Venugopalan, Michael W. Berns, Bruce J. Tromberg
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Proceedings Volume 3260, Optical Investigations of Cells In Vitro and In Vivo; (1998) https://doi.org/10.1117/12.307113
Event: BiOS '98 International Biomedical Optics Symposium, 1998, San Jose, CA, United States
Abstract
Highly focused pulsed laser microbeams can be used to precisely dissect, inactivate, or perturb cells and subcellular targets. Here we introduce a new technique which employs pulsed microbeams to transiently permeabilize the plasma cell membrane and affect the delivery of molecules from the extracellular environment into the cell. This optically produced cell permeabilization can be applied using non- specific or specific modalities. In the non-specific modality, which we term 'optoporation,' the pulsed microbeam is focused onto the glass coverslip on which the cells are plated. The generation of mechanical transients in connection with irradiation of the glass achieves molecular delivery to a number of cells proximal to the irradiation site. In the specific modality, termed 'optoinjection,' the microbeam is focused directly onto the plasma cell membrane and achieves molecular delivery into that cell alone. To quantify the irradiation geometry involved in these and other microbeam processes, as well as examine the possibility of certain non- linear effects, we have developed a system using photochromic polymer films to characterize microbeam propagation and its effects within microirradiated targets. These photochromic polymers confirm that the laser microbeam are indeed focused to submicron dimensions within the targets in our systems. In addition the behavior of such polymers at higher pulse energies and irradiances indicate that multiphoton absorption and/or plasma formation may mediate some laser microirradiation processes.
© (1998) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
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Tatiana B. Krasieva, Curtis F. Chapman, Vickie J. LaMorte, Vasan Venugopalan, Michael W. Berns, and Bruce J. Tromberg "Cell permeabilization and molecular transport by laser microirradiation", Proc. SPIE 3260, Optical Investigations of Cells In Vitro and In Vivo, (29 April 1998); https://doi.org/10.1117/12.307113
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KEYWORDS
Plasma
Polymers
Confocal microscopy
Microscopes
Pulsed laser operation
Absorption
Molecular lasers
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