Gold island films as biocompatible SERS substrates for imaging of the intracellular distribution of anticancer drugs

Ganesh D. Sockalingum; Abdelilah Beljebbar; Hamid Morjani; Michel Manfait

doi:10.1117/12.307083

29 April 1998 Gold island films as biocompatible SERS substrates for imaging of the intracellular distribution of anticancer drugs

Ganesh D. Sockalingum, Abdelilah Beljebbar, Hamid Morjani, Michel Manfait

Author Affiliations +

Proceedings Volume 3260, Optical Investigations of Cells In Vitro and In Vivo; (1998) https://doi.org/10.1117/12.307083
Event: BiOS '98 International Biomedical Optics Symposium, 1998, San Jose, CA, United States

Abstract

Highly reproducible and stable surface gold island films exhibiting long-range enhancement have been investigated and characterized as compatible for biological systems. These surface-enhanced Raman scattering (SERS) substrates allowed the non-invasive detection of micromolar concentrations of antitumor drugs using red and near-infrared excitations. Thus, good quality SERS spectra of dimethylcrocetin (DMCRT) in a single living HL60 cell have been recorded on these substrates using red excitation, without any noticeable perturbation of the cell integrity. Comparison of these spectra with FT-Raman data obtained in HL60 cells on one hand, and with FT-SERS data of the DMCRT-retinoic acid receptor (RAR) complex on the other, shows practically the same spectral profiles. However, it should be noted that with the red laser the spectrum gives additional information on the cellular components. Similarity between the signal of DMCRT-treated K562 cells and the free drug is explained by either an absence of RAR in this cell line or a lack of binding.

Citation Download Citation

Ganesh D. Sockalingum, Abdelilah Beljebbar, Hamid Morjani, and Michel Manfait "Gold island films as biocompatible SERS substrates for imaging of the intracellular distribution of anticancer drugs", Proc. SPIE 3260, Optical Investigations of Cells In Vitro and In Vivo, (29 April 1998); https://doi.org/10.1117/12.307083

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