Hyperspectral Raman imaging of bone growth and regrowth chemistry

Jerilyn A. Pezzuti; Michael D. Morris; Jeffrey F. Bonadio; Steven A. Goldstein

doi:10.1117/12.310566

9 June 1998 Hyperspectral Raman imaging of bone growth and regrowth chemistry

Jerilyn A. Pezzuti, Michael D. Morris, Jeffrey F. Bonadio, Steven A. Goldstein

Proceedings Volume 3261, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing V; (1998) https://doi.org/10.1117/12.310566
Event: BiOS '98 International Biomedical Optics Symposium, 1998, San Jose, CA, United States

Abstract

Hyperspectral Raman microscopic imaging of carbonated hydroxyapatite (HAP) is used to follow the chemistry of bone growth and regrowth. Deep red excitation is employed to minimize protein fluorescence interference. A passive line generator based on Powell lens optics and a motorized translation stage provide the imaging capabilities. Raman image contrast is generated from several lines of the HAP Raman spectrum, primarily the PO₄^-3. Factor analysis is used to minimize the integration time needed for acceptable contrast and to explore the chemical species within the bone. Bone age is visualized as variations in image intensity. High definition, high resolution images of newly formed bone and mature bone are compared qualitatively. The technique is currently under evaluation for study of experimental therapies for fracture repair.

Citation Download Citation

Jerilyn A. Pezzuti, Michael D. Morris, Jeffrey F. Bonadio, and Steven A. Goldstein "Hyperspectral Raman imaging of bone growth and regrowth chemistry", Proc. SPIE 3261, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing V, (9 June 1998); https://doi.org/10.1117/12.310566

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