9 June 1998 Three-dimensional reconstruction by focus sensing from conventional two-dimensional image slices
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Proceedings Volume 3261, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing V; (1998) https://doi.org/10.1117/12.310543
Event: BiOS '98 International Biomedical Optics Symposium, 1998, San Jose, CA, United States
Abstract
We introduce an optical means of reconstructing the topology of rough objects under the microscope or at a relatively high magnification. First, 2D images were obtained under the conventional optical microscope or under the macroscopic imaging system, focused on differing height of the sample. The depth information was extracted by sensing the focus of each pixel of the conventional image slice. Each sample height associated with the best 'focus figure' for each pixel was marked and were later utilized to generate the 3D coordinate map.
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Kie Bong Nahm, Kie Bong Nahm, Eun S. Shin, Eun S. Shin, Seok-moon Ryoo, Seok-moon Ryoo, } "Three-dimensional reconstruction by focus sensing from conventional two-dimensional image slices", Proc. SPIE 3261, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing V, (9 June 1998); doi: 10.1117/12.310543; https://doi.org/10.1117/12.310543
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