Probing the heterogeneous fluorescence-lifetime behavior of single rhodamine-6G molecules on fused silica

W. Patrick Ambrose; Peter M. Goodwin; Joerg Enderlein; David J. Semin; Richard A. Keller

doi:10.1117/12.308379

15 May 1998 Probing the heterogeneous fluorescence-lifetime behavior of single rhodamine-6G molecules on fused silica

W. Patrick Ambrose, Peter M. Goodwin, Joerg Enderlein, David J. Semin, Richard A. Keller

Proceedings Volume 3270, Methods for Ultrasensitive Detection; (1998) https://doi.org/10.1117/12.308379
Event: Optoelectronics and High-Power Lasers and Applications, 1998, San Jose, CA, United States

Abstract

Heterogeneity in the environments of molecules affects their photophysical parameters. Changes in the local environments or changes within the molecules themselves produces fluctuations in the photophysical parameters. We have explored the fluorescence lifetime and intensity fluctuations of Rhodamine 6G on silica using time correlated single photon counting combined with near-field and far- field scanning optical microscopies, and fluorescence photon-pair correlation measurements in far-field microscopy. In the most recent work, we find complex behavior in the fluorescence lifetime and emission intensity in the millisecond to minute time range that must be associated with changes in both the non-radiative and the radiative decay rates.

Citation Download Citation

W. Patrick Ambrose, Peter M. Goodwin, Joerg Enderlein, David J. Semin, and Richard A. Keller "Probing the heterogeneous fluorescence-lifetime behavior of single rhodamine-6G molecules on fused silica", Proc. SPIE 3270, Methods for Ultrasensitive Detection, (15 May 1998); https://doi.org/10.1117/12.308379

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available