15 May 1998 Sensitive on-column absorbance detection of native molecules
Author Affiliations +
Laser wave mixing is presented as a sensitive detection method for absorbance measurements in flowing liquid analytes. Wave mixing is an unusually sensitive multi-photon nonlinear optical method since the analytical signal is generated as a coherent laser beam. Since the bright signal is visible to the naked eye, optical alignment is convenient. For liquid analytes in continuously flowing cells, we have demonstrated excellent detection sensitivity levels using various wave-mixing optical configurations and laser sources. Since it is an optical absorption method, laser wave-mixing detection offers excellent detection sensitivity for both fluorescing and non-fluorescing analytes. Hence, one does not have to label non-fluorescing analytes with tags in order to obtain good detection sensitivity in wave-mixing detection methods. Sensitivity detection of analytes in their native form offers many obvious advantages especially when interfaced to popular capillary separation methods. Since the analyte laser probe volume is very small, wave-mixing detection is suitable for on-column detection in various capillary electrophoresis or micro liquid chromatography systems.
© (1998) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Sandrine Berniolles, Sandrine Berniolles, Hongjing Kan, Hongjing Kan, Lu Dai, Lu Dai, James E. Knittle, James E. Knittle, William M. Tong, William M. Tong, } "Sensitive on-column absorbance detection of native molecules", Proc. SPIE 3270, Methods for Ultrasensitive Detection, (15 May 1998); doi: 10.1117/12.308380; https://doi.org/10.1117/12.308380

Back to Top