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24 April 1998 Probing biological systems with near-field optics
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Abstract
The imaging characteristics of cantilevered NSOM probes operating in a tapping-mode feedback arrangement are discussed and compared to conventional tips employing the shear-force feedback method. Images form a wide range of samples are presented to demonstrate the surface tracking capabilities over both high and low topology samples, in addition to the low fluorescence detection limits possible utilizing the new tips. The results show that the cantilevered tip operating in a tapping-mode arrangement offers enhanced force imaging of the sample topology without compromising the low detection limits or high spatial resolution of the NSOM fluorescence images. The examples discussed here indicate that the new design will be particularly useful for applications involving biological samples that frequently exhibit complex surface topologies.
© (1998) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Hitoshi Shiku, Christopher W. Hollars, M. Annie Lee, Chad E. Talley, Greg Cooksey, and Robert C. Dunn "Probing biological systems with near-field optics", Proc. SPIE 3273, Laser Techniques for Condensed-Phase and Biological Systems, (24 April 1998); https://doi.org/10.1117/12.306124
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