Manipulation of micro-objects by means of a focused laser beam

Alexandr Jonas; Libor Sramek; Miroslav Liska; Pavel Zemanek

doi:10.1117/12.301324

1 January 1998 Manipulation of micro-objects by means of a focused laser beam

Alexandr Jonas, Libor Sramek, Miroslav Liska, Pavel Zemanek

Proceedings Volume 3320, Tenth Polish-Czech-Slovak Optical Conference: Wave and Quantum Aspects of Contemporary Optics; (1998) https://doi.org/10.1117/12.301324
Event: Tenth Polish-Czech-Slovak Optical Conference: Wave and Quantum Aspects of Contemporary Optics, 1996, Karpacz, Poland

Abstract

Force exerted on a micro-object by the focused laser beam can be as high as the gravity force acting on the object or even significantly higher. This implies that the laser light can be used to manipulate small objects (range size approximately 0.1 - 100 micrometer) within suitable immersion medium. This possibility is of a great practical importance, for example, for microbiology and molecular biology (manipulation with single living cells, cell organelles, chromosomes, etc.) as well as for micromachinery and other technical branches. We use a ray-optics-based model to determine the magnitude of forces exerted by laser light as the functions of laser beam, object and surrounding medium parameters. We study the influence of these parameters on total force in order to find the optimal parameter combination for the most effective manipulation. We have employed these theoretical results in practice and succeeded in building up a 3-D laser trap which we use to manipulate divinylbenzen spherical particles (10 - 35 micrometer sized) and also irregularly shaped living protozoa cells in water medium.

Citation Download Citation

Alexandr Jonas, Libor Sramek, Miroslav Liska, and Pavel Zemanek "Manipulation of micro-objects by means of a focused laser beam", Proc. SPIE 3320, Tenth Polish-Czech-Slovak Optical Conference: Wave and Quantum Aspects of Contemporary Optics, (1 January 1998); https://doi.org/10.1117/12.301324

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KEYWORDS

Optical spheres

Particles

Electromagnetism

Refractive index

Geometrical optics

Microscopes

Scattering

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