Anti-tumor effect of photochemical treatment by an optical parametric oscillator (OPO) laser with two photosensitizers administrations for trans-planted tumor of cultivated murine leukemia (L5178Y)

Norio Miyoshi; Zhao-Hui Jin; Kazumori Ishiguro; Masanobu Kumakiri; Keigo Takaoka; Takeshi Udagawa; Keichi Ueda; Masaru Fukuda

doi:10.1117/12.330181

2 November 1998 Anti-tumor effect of photochemical treatment by an optical parametric oscillator (OPO) laser with two photosensitizers administrations for trans-planted tumor of cultivated murine leukemia (L5178Y)

Norio Miyoshi, Zhao-Hui Jin, Kazumori Ishiguro, Masanobu Kumakiri, Keigo Takaoka, Takeshi Udagawa, Keichi Ueda, Masaru Fukuda

Author Affiliations +

Proceedings Volume 3344, 1997 Shanghai International Conference on Laser Medicine and Surgery; (1998) https://doi.org/10.1117/12.330181
Event: 1997 Shanghai International Conference on Laser Medicine and Surgery, 1997, Shanghai, China

Abstract

In order to estimate the best concentrations of two photosensitizers and the dos of laser each lights for the anti-tumor effect of these photochemical combined treaments. We examined of anti-tumor effect of the photocheical treatment by optical parametric oscillator (OPO) laser lights with two photosensitizers administrations for the transplanted tumor of a cultivated murine leukemia (L5 178Y) cells to Balb/c mude mouse because there is an advantageousness of tunable wavelengths in the OPO laser. Different two photosensitizers of a 2.5ig/gbw pheophorbide-a derivative (PH-1126) and 1mM 5-aminolevulinic acid (5-ALA) as a protoporphyrin IX (Pp-IX) precursor were used when both sensitizers were administrated together. The tumor sizes, the survival fraction and the tumor histological changes of the nude mice were observed after the treatments. From those results, it was found that (1) it was observed the anti-tumor effect by PH-1126 also in the case of the single irrdiation at even 630nm of 5-ALA absorption peak, (2) the combined irradiations at 630nm and 6SOmn for the double administrations were obtained the best anti-tumor effect of the deepened necrosis and pyknosis or disappear-changes of nuclear of the tumor cells about 1 -1.5cm at the depth 1 weak after the laser treatments comparing that of the single treatment for the single photosensitizer. Key words:OPO-laser, two different wavelength irradiations, two different photosensitizers, anti-tumor effect of L5178Y, growth curve, survival fraction

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Norio Miyoshi, Zhao-Hui Jin, Kazumori Ishiguro, Masanobu Kumakiri, Keigo Takaoka, Takeshi Udagawa, Keichi Ueda, and Masaru Fukuda "Anti-tumor effect of photochemical treatment by an optical parametric oscillator (OPO) laser with two photosensitizers administrations for trans-planted tumor of cultivated murine leukemia (L5178Y)", Proc. SPIE 3344, 1997 Shanghai International Conference on Laser Medicine and Surgery, (2 November 1998); https://doi.org/10.1117/12.330181

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KEYWORDS

Tumors

Optical parametric oscillators

Leukemia

Skin

Tunable lasers

Laser irradiation

Tissue optics

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