Release of thromboxane by photodynamically activated rat platelets

Chuannong Zhou; Geng-Hong Zhou; Jinsheng Deng; Junlin Liang; Wen-Kai Liu

doi:10.1117/12.330175

2 November 1998 Release of thromboxane by photodynamically activated rat platelets

Chuannong Zhou, Geng-Hong Zhou, Jinsheng Deng, Junlin Liang, Wen-Kai Liu

Proceedings Volume 3344, 1997 Shanghai International Conference on Laser Medicine and Surgery; (1998) https://doi.org/10.1117/12.330175
Event: 1997 Shanghai International Conference on Laser Medicine and Surgery, 1997, Shanghai, China

Abstract

The purpose of this study is to explore the significance of photodynamic activation of platelets in the mechanism of photodynamic destruction of tumors. Normal rats were divided into control and experimental groups, 6 rats in each group. Hematoporphyrin derivative was given in a dose of 10 or 20 mg/kg iv. Blood was taken and plasma rich in platelets was obtained by centrifugation. HeNe laser irradiation of the platelets in plasma was performed ex vivo at 30 mW, spot diameter of 6 mm for 16 or 32 minutes (100 or 200 J/cm2). Aspirin was given in one group in a dose of20 mg/kg p.o.at 24 and 3hrs prior to irradiation, while indomethacin was given in another group in a dose of 0.5 mg/kg. The mean± standard diviation of TXB2 and 6-keto-PGF-lcxconcentration in the plasma, and the TXB2/6-keto-PGF-la ratio were calculated for each group and compared statistically between groups by Student's t-test. The results showed that the TXA2 release was tending to increase and TXB2/6-keto-PGF-la ratio was significantly increased after PDT treatment in a dose-response relationship. Our findings evidently support that platelet activation may play an important role in the mechanism of microvascular damage, intravascular clotting and microcirculatory disturbance induced by photodynamic therapy. Indomethacin showed a more strong inhibitory effect on this process than aspirin did. It indicates that in patients taking indomethacin or aspirin, photodynamic therapy should be performed a few day after stopping those drugs. Key words: Photodynamic therapy, Platelets, Thromboxanes, Prostacyclins

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Chuannong Zhou, Geng-Hong Zhou, Jinsheng Deng, Junlin Liang, and Wen-Kai Liu "Release of thromboxane by photodynamically activated rat platelets", Proc. SPIE 3344, 1997 Shanghai International Conference on Laser Medicine and Surgery, (2 November 1998); https://doi.org/10.1117/12.330175

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KEYWORDS

Photodynamic therapy

Plasma

Helium neon lasers

Tumors

Blood

In vivo imaging

Blood vessels

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