26 May 1998 Laser-induced saturation of fluorescence for complex organic molecules
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Proceedings Volume 3404, ALT'97 International Conference on Laser Surface Processing; (1998) https://doi.org/10.1117/12.308644
Event: ALT '97 International Conference on Laser Surface Processing, 1997, Limoges, France
Abstract
The effect of fluorescence saturation is caused by limited life-time of molecules in excited state, and appears as a non-linear dependence of detected fluorescence signal versus excitation intensity under laser pulsed irradiation of the sample. It is noticeable at conditions of 1024 exciting photons per second per square centimeter, which we usually have for laboratory laser fluorimeter. Spectroscopy of saturated fluorescence is a promising tool for studying such spectral molecular characteristics, as fluorescence life- time, absorption cross-section, constant of singlet-singlet annihilation, and their changes due to molecular interaction. The work presents the theory of the effect considering the cases of different intermolecular interaction (quenching mechanisms, singlet-singlet annihilation) and describes the experimental results on florescence saturation for several organic dyes in water.
© (1998) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Victor I. Yuzhakov, Victor I. Yuzhakov, K. G. Yevsyukhina, K. G. Yevsyukhina, Svetlana V. Patsayeva, Svetlana V. Patsayeva, } "Laser-induced saturation of fluorescence for complex organic molecules", Proc. SPIE 3404, ALT'97 International Conference on Laser Surface Processing, (26 May 1998); doi: 10.1117/12.308644; https://doi.org/10.1117/12.308644
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