UV laser radiosensitivity of normal and tumoral chromatin

Liliana Radu; Ion N. Mihailescu; V. Preoteasa; Irina Radulescu; Doina Gostian; Alexandru Hening; S. Radu

doi:10.1117/12.312827

2 July 1998 UV laser radiosensitivity of normal and tumoral chromatin

Liliana Radu, Ion N. Mihailescu, V. Preoteasa, Irina Radulescu, Doina Gostian, Alexandru Hening, S. Radu

Proceedings Volume 3405, ROMOPTO '97: Fifth Conference on Optics; (1998) https://doi.org/10.1117/12.312827
Event: ROMOPTO '97: Fifth Conference on Optics, 1997, Bucharest, Romania

Abstract

A normal and a tumoral chromatin radiosensitivity to an UV laser radiation was determined. The characteristics of these chromatin samples were established by the analysis of the absorption and emission spectra of chromatin complexes with a specific DNA ligand-ethidium bromide, by Scatchard representations of ligand binding to chromatin and by the Forster energy transfer efficiency determination between two fluorescent ligands coupled at chromatin: dansyl chloride and acridine orange. The effects of excimer laser beam with (lambda) equals 248 nm on chromatin structure were analyzed by the above methods and also by the establishment of the intrinsic chromatin fluorescence and of the excited state lifetimes of a specific DNA ligand.

Citation Download Citation

Liliana Radu, Ion N. Mihailescu, V. Preoteasa, Irina Radulescu, Doina Gostian, Alexandru Hening, and S. Radu "UV laser radiosensitivity of normal and tumoral chromatin", Proc. SPIE 3405, ROMOPTO '97: Fifth Conference on Optics, (2 July 1998); https://doi.org/10.1117/12.312827

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