Statistical methods for 3D reconstruction of viruses using cryo electron microscopy data

Wen Gao; Peter C. Doerschuk

doi:10.1117/12.323797

22 September 1998 Statistical methods for 3D reconstruction of viruses using cryo electron microscopy data

Wen Gao, Peter C. Doerschuk

Proceedings Volume 3459, Bayesian Inference for Inverse Problems; (1998) https://doi.org/10.1117/12.323797
Event: SPIE's International Symposium on Optical Science, Engineering, and Instrumentation, 1998, San Diego, CA, United States

Abstract

Cryo electron microscopy of viruses provides 2D projections of the scattering intensity of the viral particle but the orientation of the projections is not known. We describe an approach to reconstructing the 3D scattering intensity in spite of the unknown projection orientations using nonlinear least squares ideas where the reconstruction is guaranteed to have the icosahedral symmetry known to be present in the viral particle.

Citation Download Citation

Wen Gao and Peter C. Doerschuk "Statistical methods for 3D reconstruction of viruses using cryo electron microscopy data", Proc. SPIE 3459, Bayesian Inference for Inverse Problems, (22 September 1998); https://doi.org/10.1117/12.323797

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available