Microbial analysis using Sharma's reaction

Zulfiqur Ali; Bruce Hodson; James Timmins; Stephen Keith

doi:10.1117/12.339046

10 February 1999 Microbial analysis using Sharma's reaction

Zulfiqur Ali, Bruce Hodson, James Timmins, Stephen Keith

Author Affiliations +

Proceedings Volume 3534, Environmental Monitoring and Remediation Technologies; (1999) https://doi.org/10.1117/12.339046
Event: Photonics East (ISAM, VVDC, IEMB), 1998, Boston, MA, United States

Abstract

The photoreaction of thionine with NAD(P)H leads to the formation of non-fluorescent semi and lecuothionine. This has been previously used for the development of enzymatic assays. We show that the fluorescence of thionine is quenched in the presence of Nocardia corallin. We ascribe this decrease in fluorescence to the presence of NAD(P)H present on the walls and in the body of the cells as described by Sharma. We therefore demonstrate that it is possible to measure, in situ, NAD(P)H in Nocardia corallin in a simple and inexpensive manner and that this finding extends the analytical application of Sharma's reaction to other organisms.

Citation Download Citation

Zulfiqur Ali, Bruce Hodson, James Timmins, and Stephen Keith "Microbial analysis using Sharma's reaction", Proc. SPIE 3534, Environmental Monitoring and Remediation Technologies, (10 February 1999); https://doi.org/10.1117/12.339046

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

;

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE