18 December 1998 Waveguide immunofluorescence sensor for water pollution analysis
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Proceedings Volume 3539, Chemical Microsensors and Applications; (1998) https://doi.org/10.1117/12.333751
Event: Photonics East (ISAM, VVDC, IEMB), 1998, Boston, MA, United States
Abstract
A regenerable channel waveguide fluorescence sensor for environmental monitoring is reported. The sensor has been characterized as a detector of the pesticide 2,4 dichlorophenoxyacetic acid. A binding inhibition assay, using fluorescent Cy5.5 dye-labeled antibodies, was monitored at the modified surface of the glass waveguide to detect the target analyte. Three calibration curves were determined and averaged. The averaged calibration curve has a mid-point of 0.68 ppb and a calculated detection limit of 0.28 ppb. Incorporation of a 20 nm thick tantalum pentoxide film at the waveguide surface enhanced the peak fluorescence signal by a factor of approximately 6 compared with an uncoated sensor.
© (1998) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Richard D. Harris, G. R. Quigley, James S. Wilkinson, Albrecht Klotz, C. Barzen, Andreas Brecht, Guenter Gauglitz, R. A. Abuknesha, "Waveguide immunofluorescence sensor for water pollution analysis", Proc. SPIE 3539, Chemical Microsensors and Applications, (18 December 1998); doi: 10.1117/12.333751; https://doi.org/10.1117/12.333751
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