Automated filtration capture immunoelectrochemical assay of bacteria

Jeffrey D. Brewster

doi:10.1117/12.335767

12 January 1999 Automated filtration capture immunoelectrochemical assay of bacteria

Jeffrey D. Brewster

Proceedings Volume 3544, Pathogen Detection and Remediation for Safe Eating; (1999) https://doi.org/10.1117/12.335767
Event: Photonics East (ISAM, VVDC, IEMB), 1998, Boston, MA, United States

Abstract

An automated system for filtration capture and immunoelectrochemical detection of bactria in liquid samples is described. The detector incorporates a porous electrode in contact with the filter, rather than the solid electrode used previously, to allow sample and reagent solutions to be delivered in a flowing stream. This eliminated the need for manual assembly of the electrode and filter for each assay and allowed repetitive assays on a single filter/electrode. The electrochemical response of the novel gold grid electrode under static and flow conditions was found to be consistent with theory for a planar electrode operating in laminar flow conditions. A computer-controlled fluid handling system was coupled to the detector for delivery of samples and reagents at controlled flow rates and times. The combination of flow detector and fluid handling system allows for automation of the previous assay protocol as well as providing new operating modes with enhanced background rejection and improved sensitivity. The use of these operating modes is demonstrated by a simple assay for Escherichia coli O157:H7 with virtually no background current.

Citation Download Citation

Jeffrey D. Brewster "Automated filtration capture immunoelectrochemical assay of bacteria", Proc. SPIE 3544, Pathogen Detection and Remediation for Safe Eating, (12 January 1999); https://doi.org/10.1117/12.335767

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