7 August 1998 Visualization of molecule interaction between antigen and antibody: one of the ellipsometric imaging applications
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It is to investigate molecule interactions between antigen and antibody with ellipsometric imaging technique and demonstrate some features and possibilities offered by applications of the technique. Molecule interaction is an important interest for molecule biologist and immunologist. They have used some established methods such as immunofluorescence, radioimmunoassay and surface plasma resonance, etc. to study the molecule interaction. At the same time, experimentalists hope to use some updated technique with more direct visual results. Ellipsometric imaging is non-destructive and exhibits a high sensitivity to phase transitions with thin layers. It is capable of imaging local variations in the optical properties such as thickness due to the presence of different surface concentration of molecule or different deposited molecules. If a molecular mono-layer (such as antigen) with bio-activity were deposited on a surface to form a sensing surface and then incubated in a solution with other molecules (such as antibody), a variation of the layer thickness when the molecules on the sensing surface reacted with the others in the solution could be observed with ellipsometric imaging. Every point on the surface was measured at the same time with a high sensitivity to distinguish the variation between mono- layer and molecular complexes. Ellipsometric imaging is based on conventional ellipsometry with charge coupled device (CCD) as detector and images are caught with computer with image processing technique. It has advantages of high sensitivity to thickness variation (resolution in the order of angstrom), big field of view (in square centimeter), high sampling speed (a picture taken within one second), and high lateral resolution (in the order of micrometer). Here it has just shown one application in study of antigen-antibody interaction, and it is possible to observe molecule interaction process with an in-situ technique.
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Gang Jin, Gang Jin, Yonghong Meng, Yonghong Meng, Jianhua Xing, Jianhua Xing, Ziyan Zhao, Ziyan Zhao, "Visualization of molecule interaction between antigen and antibody: one of the ellipsometric imaging applications", Proc. SPIE 3548, Biomedical Optics and Lasers: Diagnostics and Treatment, (7 August 1998); doi: 10.1117/12.317855; https://doi.org/10.1117/12.317855


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