3 February 1999 PDT-induced apoptosis in bladder carcinoma cells
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Abstract
Photodynamic therapy (PDT) is a highly efficient inducer of apoptosis in EY-28 bladder carcinoma cells, resulting in extensive DNA fragmentation. Bladder carcinoma cells EY-28 (Tumorbank Heidelberg, Germany) were incubated for 1 h with 1 (mu) g AamTPPn/ml or 2 (mu) g AamTPPn/ml. After incubation cells were refed with complete medium and irradiated with 0.75 J/cm2. To identify apoptotic cells, a in situ cell death detection kit POD (Boehringer Mannheim, Germany) was used. The chromatin condensation characteristic to apoptotic cells was detected by transmission electron microscopy. Using 1 (mu) g AamTPPn/ml and 2 (mu) g AamTPPn/ml (9-Acetamido-2,7,12,17- tetra-n-Porpylporphycene), respectively, and irradiation at 0.75 J/cm2, a percentage of 36.9% and 54.7%, respectively, of apoptotic cells was detected.
© (1999) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Ruediger Bachor, Ruediger Bachor, Ella Dumbravia Reich, Ella Dumbravia Reich, Klaus Kleinschmidt, Klaus Kleinschmidt, Denes Repassy, Denes Repassy, Richard E. Hautmann, Richard E. Hautmann, } "PDT-induced apoptosis in bladder carcinoma cells", Proc. SPIE 3563, Photochemotherapy of Cancer and Other Diseases, (3 February 1999); doi: 10.1117/12.339132; https://doi.org/10.1117/12.339132
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