Translator Disclaimer
3 February 1999 PDT-induced apoptosis in bladder carcinoma cells
Author Affiliations +
Photodynamic therapy (PDT) is a highly efficient inducer of apoptosis in EY-28 bladder carcinoma cells, resulting in extensive DNA fragmentation. Bladder carcinoma cells EY-28 (Tumorbank Heidelberg, Germany) were incubated for 1 h with 1 (mu) g AamTPPn/ml or 2 (mu) g AamTPPn/ml. After incubation cells were refed with complete medium and irradiated with 0.75 J/cm2. To identify apoptotic cells, a in situ cell death detection kit POD (Boehringer Mannheim, Germany) was used. The chromatin condensation characteristic to apoptotic cells was detected by transmission electron microscopy. Using 1 (mu) g AamTPPn/ml and 2 (mu) g AamTPPn/ml (9-Acetamido-2,7,12,17- tetra-n-Porpylporphycene), respectively, and irradiation at 0.75 J/cm2, a percentage of 36.9% and 54.7%, respectively, of apoptotic cells was detected.
© (1999) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Ruediger Bachor, Ella Dumbravia Reich, Klaus Kleinschmidt, Denes Repassy, and Richard E. Hautmann "PDT-induced apoptosis in bladder carcinoma cells", Proc. SPIE 3563, Photochemotherapy of Cancer and Other Diseases, (3 February 1999);


Thomas J. Dougherty: present at the creation
Proceedings of SPIE (August 07 2019)
Photodynamic therapy of human tubulo-villous adenomas
Proceedings of SPIE (January 12 1995)
PDT: death pathways
Proceedings of SPIE (February 09 2007)
PDT of bladder carcinoma: the old and the new
Proceedings of SPIE (July 13 2004)

Back to Top