30 December 1998 Measurement of in-vivo hemoglobin concentration using diffuse reflectance
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A means of determining the concentration of hemoglobin in- vivo using diffuse reflectance is proposed which could be applied to the quantification of laser Doppler flowmetry data. Monte Carlo simulations were used to generate intensity vs. source-detector separation plots for optical fibers of 200 micrometers diameter, with separations up to 6 mm on the skin surface. The plots were then fitted to the equation; I(x) equals a0.exp(-a1x + a2x2) and a polynomial function derived for a1 that is dependent upon the reduced scattering, microsecond(s) ', and absorption, (mu) a, coefficients. Experimentally two wavelengths with a known difference in the absorption coefficient of hemoglobin, but sharing the same microsecond(s) ', are used to solve simultaneously the derived function for (mu) a, and give the hemoglobin concentration.
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Ashley D. Hunter, John A. Crowe, John G. Walker, "Measurement of in-vivo hemoglobin concentration using diffuse reflectance", Proc. SPIE 3566, Photon Propagation in Tissues IV, (30 December 1998); doi: 10.1117/12.334355; https://doi.org/10.1117/12.334355

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